PerlPrimer 1.1.14

Operating systemsOS : Linux / BSD
Program licensingScript Licensing : GPL - GNU Public License
CreatedCreated : Jun 7, 2007
Size downloadDownloads : 3
Program licensing
Thank you for voting...

PerlPrimer is a free, open-source GUI application ...

perlprimer by Owen Marshall is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite pcr, real-time PCR (qpcr) and sequencing. It aims to automate and simplify the process of primer design. PerlPrimer 1.1.14's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cdna sequences from Ensembl(including both sequences automatically for QPCR)
- ability to BLAST search primers using the NCBI server or a local server
- Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted to be in-frame
- QPCR primer design without manual intron-exon boundary entry
PerlPrimer [perlprimer1.1.14.exe] calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. It allows the user to specify the concentration of Mg2 , dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.

PerlPrimer 1.1.14 scripting tags: temperature, server, perlprimer, informatics, sequences, software, pcr, qpcr, bio informatics tool, bio informatics software, ability, bio informatics utility, primers. What is new in PerlPrimer 1.1.14 software script? - Unable to find PerlPrimer 1.1.14 news. What is improvements are expecting? Newly-made PerlPrimer 1.2 will be downloaded from here. You may download directly. Please write the reviews of the PerlPrimer. License limitations are unspecified.